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Circular RNAs in monkey muscle: age-dependent changes.

Kotb Abdelmohsen1, Amaresh C Panda1, Supriyo De1

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Aging
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Summary
This summary is machine-generated.

Researchers identified thousands of circular RNAs (circRNAs) in Rhesus macaque skeletal muscle. Some circRNAs change with age, potentially impacting muscle function.

Keywords:
RNA-sequencingagingpost-transcriptional gene regulationribonucleoprotein complexsplicing

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Area of Science:

  • Molecular Biology
  • Genomics
  • Muscle Physiology

Background:

  • Circular RNAs (circRNAs) are a class of RNA molecules known to regulate gene expression, often by interacting with microRNAs.
  • Previous studies have identified circRNAs in various species, but their role in primate skeletal muscle remains largely unexplored.

Purpose of the Study:

  • To identify and characterize circular RNAs (circRNAs) in Rhesus macaque skeletal muscle.
  • To investigate the age-dependent changes in circRNA expression in primate skeletal muscle.
  • To explore the potential functional implications of identified circRNAs in muscle aging.

Main Methods:

  • RNA sequencing (RNA-seq) was used to identify and annotate circRNAs, including circular intronic RNAs (ciRNAs), in skeletal muscle tissue from Rhesus macaques of varying ages.
  • Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was employed to validate the presence and abundance of selected circRNAs.

Main Results:

  • Approximately 12,000 circRNAs were identified and annotated in Rhesus macaque skeletal muscle.
  • Several highly abundant circRNAs were detected, and a subset showed differential expression correlated with advancing age.
  • The presence and abundance of specific circRNAs were confirmed using RT-qPCR.

Conclusions:

  • This study provides a systematic catalog of circRNAs expressed in primate skeletal muscle.
  • Age-associated changes in circRNA abundance suggest their potential involvement in the aging process of skeletal muscle.
  • Further research is warranted to elucidate the specific functions of these circRNAs in maintaining muscle health and function.