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Published on: April 1, 2019
Association of Tumor Necrosis Factor Alpha 308 G/A Polymorphism with Asthma in Pakistani Population
Nusrat Saba1, Osman Yusuf2, Sadia Rehman3
1Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan AND Department of Biochemistry, PirMehar Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
Abstract:
Asthma is a chronic inflammatory and remodeling disorder of the airways, in which many cells, cellular elements, and cytokines play important roles. The role of tumor necrosis factor-α (TNF-α) in asthma is unclear in Pakistani population. The aim of this study was to assess the relationship between TNF-α-308 polymorphism and asthma. Polymorphism of TNF-α (G-308-A locus; rs 1800629) in 329 asthmatic patients and 151 healthy controls was evaluated. DNA was prepared from blood samples of cases and controls. Samples were genotyped for TNF-α 308 G/A polymorphism. There was no significant difference in the frequency of GG (OR 1.049 with 95% CI 0.68-1.63) and GA (OR 0.987 with 95% CI 0.64-1.53) genotypes of TNF-α-308. The AA genotype was absent in cases and only one AA genotype was observed in the controls. The genetic polymorphism of TNF-α does not seem to be associated with asthma in Pakistani population.
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