HEPATOPROTECTIVE ACTIVITY OF EXOGENOUS RNA.

Area of Science:

• Pharmacology
• Hepatology
• Biochemistry

Background:

• Thioacetamide-induced hepatotoxicity is a common model for studying liver injury.
• Investigating novel therapeutic agents for liver protection is crucial.

Purpose of the Study:

• To evaluate the hepatoprotective activity of Nuclex, a yeast RNA-based pharmaceutical.
• To assess Nuclex's efficacy in models of acute and chronic liver injury.

Main Methods:

• Administration of Nuclex (200 mg/kg) during thioacetamide-induced acute and chronic liver injury.
• Histopathological examination of liver parenchyma for lesions and inflammatory infiltration.
• Biochemical analysis of oxidative stress markers, including TBA-reactive products, carbonyl derivatives, protein thiols, and reduced glutathione.

Main Results:

• Nuclex administration significantly reduced liver parenchyma lesions and inflammatory infiltration.
• Nuclex attenuated thioacetamide-induced free radical damage to hepatic biopolymers.
• Key indicators of oxidative stress, such as TBA-reactive products and carbonyl derivatives, were reduced, while protective molecules like protein thiols and reduced glutathione were restored.

Conclusions:

• Nuclex exhibits significant hepatoprotective activity in both acute and chronic liver injury models.
• The protective mechanism involves reducing hepatocellular damage, inflammation, and oxidative stress.