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[ACTIVITY OF Ca2+,Mg(2+)-ATPase OF SARCOPLASMIC RETICULUM AND CONTRACTION STRENGTH OF THE FROG SKELETAL MUSCLES UNDER

D M Nozdrenko, L V Korchinska, V M Soroca

    Ukrainian Biochemical Journal
    |November 10, 2015
    PubMed

    Insights

    Organophosphorus insecticides like chlorpyrifos impair frog muscle contraction and reduce Ca2+, Mg2+-ATPase enzyme activity in the sarcoplasmic reticulum. This study highlights their non-cholinergic effects on muscle function.

    Area of Science:

    • Environmental toxicology
    • Muscle physiology
    • Biochemistry

    Context:

    • Organophosphorus insecticides are widely used pesticides.
    • Their non-cholinergic effects on skeletal muscle are not fully understood.
    • Investigating the impact of specific insecticides on muscle fiber function is crucial.

    Purpose:

    • To investigate the effects of pirimiphosmethyl, diazinon, and chlorpyrifos on Rana temporaria muscle contraction.
    • To assess the impact of these insecticides on sarcoplasmic reticulum Ca2+, Mg2+-ATPase activity.
    • To determine the concentration-dependent relationship between insecticide exposure and muscle response.

    Summary:

    • Organophosphorus insecticides (pirimiphosmethyl, diazinon, chlorpyrifos) reduced contraction properties in Rana temporaria tibialis anterior muscle fiber bundles.
    • A concentration-dependent decrease in muscle strength was observed, indicating a non-cholinergic mechanism.
    • All tested insecticides inhibited sarcoplasmic reticulum Ca2+, Mg2+-ATPase activity, with chlorpyrifos showing the most significant effect.

    Impact:

    • This research reveals a novel mechanism of organophosphorus insecticide toxicity impacting muscle function.
    • Findings suggest potential risks to muscle health and performance in organisms exposed to these pesticides.
    • The study provides biochemical evidence for the detrimental effects of specific organophosphorus compounds on muscle calcium regulation.

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