Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Adalimumab-polyelectrolyte nanocomplexes decelerate synovial drug clearance <i>ex vivo</i>.

Nanoscale·2026

Same author

Spray-freeze-drying aprepitant with hydroxypropyl cellulose increases nasal bioavailability.

International journal of pharmaceutics: X·2026

Same author

Systematic investigation of thermal process parameters on the morphology of spray-freeze-dried powders.

Journal of colloid and interface science·2026

Same author

Impact of Drug Hydrophilicity on Transdermal Delivery by Nanoemulsions.

Pharmaceutics·2026

Same author

Combined extraction and spray-freeze-drying of Cannabis sativa Flos provides stable lyophilizate particles of cannabinoids.

Journal of cannabis research·2026

Same author

In-Situ Forming Polyester Implants for Sustained Intravesical Oxybutynin Release.

Pharmaceutics·2025

Search research articles

Related Experiment Video

Updated: Mar 30, 2026

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Published on: September 28, 2022

Nanoparticles as drug carriers: current issues with in vitro testing.

Maryam Alsadat Shetab Boushehri¹, Alf Lamprecht^1,2

¹Department of Pharmaceutics, Institute of Pharmacy, University of Bonn, Bonn, Germany.

Nanomedicine (London, England)

|November 10, 2015

Summary

Nanotechnology enhances drug delivery through nanocarriers, but in vitro tests for particle size, charge, release, and toxicity need improvement. Better in vitro methods are crucial for predicting in vivo nanocarrier performance and safety.

Keywords:

drug incorporation drug release in vitro assessment procedures nanocarriers particle size release kinetics surface charge toxicity

More Related Videos

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

Related Experiment Videos

Last Updated: Mar 30, 2026

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Published on: September 28, 2022

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Manufacture and Drug Delivery Applications of Silk Nanoparticles

Published on: October 8, 2016

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

Area of Science:

Nanomedicine
Materials Science
Biotechnology

Background:

Nanotechnology offers advanced nanocarriers for targeted drug delivery, improved bioavailability, and reduced side effects.
Rigorous in vitro evaluation of nanocarriers is essential before in vivo administration to ensure stability, safety, and efficacy.
Current in vitro characterization methods for nanocarriers include assessing particle size, surface charge, drug release kinetics, and toxicity.

Purpose of the Study:

To discuss current challenges and limitations in in vitro evaluation techniques for nanocarriers.
To examine the sufficiency of existing in vitro methods in predicting in vivo nanocarrier behavior.
To propose considerations for enhancing the correlation between in vitro and in vivo nanocarrier assessments.

Main Methods:

Review and critical analysis of established in vitro characterization techniques for nanocarriers.
Discussion of specific parameters: particle size, surface charge, drug release, and toxicity assays.
Exploration of the predictive power of in vitro data for in vivo outcomes.

Main Results:

In vitro methods for nanocarrier characterization present limitations in accurately predicting in vivo performance.
Current techniques may not fully capture the complex biological interactions occurring in vivo.
Significant discrepancies can exist between in vitro release profiles and actual in vivo drug disposition.

Conclusions:

In vitro evaluation of nanocarriers requires refinement to better align with in vivo realities.
Improvements in in vitro methodologies are necessary for reliable prediction of nanocarrier safety and efficacy.
Further research should focus on developing advanced in vitro models that better mimic physiological conditions for nanocarrier assessment.