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Host-Microbe Protein Interactions during Bacterial Infection.

Devin K Schweppe1, Christopher Harding2, Juan D Chavez1

  • 1Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195, USA.

Chemistry & Biology
|November 10, 2015
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Summary
This summary is machine-generated.

This study reveals how Acinetobacter baumannii proteins interact with human lung cells during infection. The key bacterial protein OmpA targets host cell adhesion structures, impacting invasion and disease.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Host-Pathogen Interactions

Background:

  • Interspecies protein-protein interactions are crucial for bacterial infections.
  • Identifying host targets and interaction structures of bacterial proteins is challenging.

Purpose of the Study:

  • To identify interspecies protein-protein interactions between Acinetobacter baumannii and human lung epithelial cells.
  • To characterize the structural basis of these interactions and their role in infection.

Main Methods:

  • Utilized chemical crosslinking coupled with mass spectrometry (MS) for unbiased identification of protein interactions.
  • Employed co-immunoprecipitation and transposon mutant experiments for validation.

Main Results:

  • Identified 46 interspecies protein-protein interactions and 3,076 crosslinked peptide pairs.
  • Found that the Acinetobacter baumannii virulence factor OmpA interacts with host desmosomal proteins.
  • Demonstrated the role of these interactions in bacterial invasion and acute lung infection.

Conclusions:

  • The study elucidates novel Acinetobacter baumannii-host protein interactions and their structural underpinnings.
  • The chemical crosslinking/MS approach is a powerful tool for studying host-pathogen interactions in various systems.