Related Experiment Video

Updated: Mar 30, 2026

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
08:57

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Published on: March 10, 2014

12.9K

The Pseudomonas aeruginosa AmrZ C-terminal domain mediates tetramerization and is required for its activator and

Binjie Xu1,2, Yue Ju3, Randal J Soukup4

  • 1Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA.

Environmental Microbiology Reports
|November 10, 2015
PubMed

Insights

The Pseudomonas aeruginosa transcription factor AmrZ primarily exists as a tetramer. Its C-terminal domain is crucial for this tetramerization, DNA binding, and regulating virulence factors.

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Pseudomonas aeruginosa is an opportunistic pathogen, particularly dangerous for cystic fibrosis patients.
  • The transcription factor AmrZ controls virulence factor expression in P. aeruginosa.
  • AmrZ is part of the ribbon-helix-helix superfamily, known for diverse oligomeric states.

Purpose of the Study:

  • To determine the oligomeric state of the AmrZ protein in solution.
  • To investigate the role of the AmrZ C-terminal domain in its function.

Main Methods:

  • Utilized four independent experimental approaches to analyze AmrZ.
  • Investigated the impact of C-terminal domain deletion on AmrZ properties.

Main Results:

  • Demonstrated that AmrZ predominantly forms tetramers in solution.
  • Showed that C-terminal deletion disrupts tetramerization and reduces DNA binding.
  • Confirmed the C-terminal domain's essential role in AmrZ-mediated gene regulation.

Conclusions:

  • The tetrameric form of AmrZ is the primary functional unit.
  • The C-terminal domain is vital for AmrZ oligomerization, DNA interaction, and virulence gene regulation.

More Related Videos

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

8.7K
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep105011:27

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Published on: May 13, 2020

4.4K

Related Concept Videos

Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
26.8K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

11.5K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.4K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
13.3K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
879
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K