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Insulin induces the expression of FGF2 but does not synergize with it during angiogenesis

Lakshmi Surekha Krishnapati1, Surendra Ghaskadbi1

  • 1Developmental Biology Group, MACS-Agharkar Research Institute, Pune 411 004, India.

Microvascular Research
|November 10, 2015
PubMed

Insights

This study investigated the interplay between insulin, fibroblast growth factor-2 (FGF-2), and vascular endothelial growth factor (VEGF) in blood vessel formation. Researchers found no evidence of a combined effect of insulin and FGF-2 on angiogenesis.

Area of Science:

  • Biomedical research
  • Molecular biology
  • Cardiovascular science

Background:

  • Cardiovascular and ischemic diseases are linked to diabetes mellitus, often caused by blocked blood vessels.
  • Current treatments involve growth factors, gene therapy, and stem cells.
  • Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) promote blood vessel growth but are elevated with insulin therapy, suggesting a potential link.

Purpose of the Study:

  • To investigate the potential synergistic effect between insulin and FGF-2 in angiogenesis.
  • To explore the combinatorial effects of insulin, FGF-2, and VEGF on blood vessel formation.

Main Methods:

  • Chick embryonic chorioallantoic membrane (CAM) assay for morphological and histological analysis.
  • Semi-quantitative reverse transcriptase PCR (RT-PCR) to analyze angiogenesis regulatory gene expression.

Main Results:

  • Morphological and histological examination using the CAM assay indicated no synergistic effect between insulin and FGF-2.
  • Gene expression analysis confirmed the absence of a combinatorial effect between insulin and FGF-2.
  • The CAM assay also demonstrated no combined effect of insulin, FGF-2, and VEGF on angiogenesis.

Conclusions:

  • Insulin and FGF-2 do not appear to have a synergistic role in promoting angiogenesis.
  • The study suggests a lack of combinatorial effect between insulin, FGF-2, and VEGF in blood vessel formation.
Keywords:
AngiogenesisFGF-2InsulinVEGF

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