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The protective effect of intraperitoneal medical ozone preconditioning and treatment on hepatotoxicity induced by

Arif Aslaner1, Tuğrul Çakır1, Betül Çelik2

  • 1Department of General Surgery, Antalya Education and Research Hospital Antalya, Turkey.

International Journal of Clinical and Experimental Medicine
|November 10, 2015
PubMed

Insights

Medical ozone effectively protected rats against methotrexate-induced liver damage. Ozone therapy reversed elevated liver enzymes and inflammatory markers, demonstrating its therapeutic potential for drug-induced hepatotoxicity.

Area of Science:

  • Toxicology
  • Pharmacology
  • Biochemistry

Background:

  • Methotrexate (MTX) is a chemotherapy agent known to cause significant liver toxicity.
  • Hepatotoxicity induced by MTX presents challenges in clinical management, necessitating protective strategies.
  • The potential of medical ozone as a protective agent against drug-induced liver injury requires investigation.

Purpose of the Study:

  • To investigate the protective effects of medical ozone preconditioning and treatment on methotrexate-induced hepatotoxicity in a rat model.
  • To evaluate the biochemical and histopathological alterations in liver tissue following MTX administration and ozone intervention.

Main Methods:

  • Eighteen rats were divided into control, MTX-induced hepatotoxicity, and MTX with ozone groups.
  • Hepatotoxicity was induced by a single dose of 20 mg/kg MTX.
  • Medical ozone was administered intraperitoneally as preconditioning and treatment in the ozone group.
  • Liver enzymes (ALT, AST), inflammatory cytokines (TNF-α, IL-1β), oxidative stress markers (MDA, GSH), and myeloperoxidase (MPO) were measured.
  • Histopathological examination assessed liver injury scores.

Main Results:

  • MTX administration significantly increased liver enzymes (ALT, AST), MDA, MPO activity, and pro-inflammatory cytokines (TNF-α, IL-1β), while decreasing GSH levels.
  • Medical ozone preconditioning and treatment significantly reversed these biochemical changes induced by MTX.
  • Histopathological examination revealed that ozone therapy ameliorated MTX-induced liver damage.

Conclusions:

  • Medical ozone demonstrates significant hepatoprotective effects against acute methotrexate-induced liver injury in rats.
  • Ozone therapy effectively mitigates oxidative stress and inflammation associated with MTX hepatotoxicity.
  • These findings suggest medical ozone as a potential therapeutic strategy for managing MTX-induced liver damage.
Keywords:
Methotrexatehepatotoxicitylivermedical ozonepreconditioning

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