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Related Experiment Video

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Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Published on: September 29, 2019

High-affinity interaction between interleukin-11 and S100P protein.

Alexei S Kazakov¹, Andrei S Sokolov¹, Victoria A Rastrygina¹

¹Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia.

Biochemical and Biophysical Research Communications

|November 10, 2015

Summary

Interleukin-11 (IL-11) and S100P proteins interact under physiological conditions, suggesting a role in cancer. This specific interaction may be a target for novel cancer therapies.

Keywords:

Cancer Interleukin Protein–protein interaction S100 protein STAT3

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Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

Interleukin-11 (IL-11) and S100P are oncoproteins frequently co-expressed in various cancers.
Their co-expression suggests a potential functional interaction during cancer development (oncogenesis).

Purpose of the Study:

To investigate the interaction between Interleukin-11 (IL-11) and S100P.
To characterize the binding affinity and conditions of this interaction.

Main Methods:

Surface plasmon resonance spectroscopy
Intrinsic fluorescence
Chemical crosslinking
Cell-based assays using HEK293 cells

Main Results:

Recombinant IL-11 and S100P were found to interact under physiological calcium ion concentrations.
IL-11 possesses at least two binding sites for S100P, with dissociation constants of 32 nM and 288 nM.
S100P binding affinity to IL-11 is 5-13 fold lower than IL-11's affinity for its receptor subunit alpha.
S100P did not affect IL-11-induced STAT3 activation in HEK293 cells but may influence other oncogenic pathways.

Conclusions:

A specific interaction between IL-11 and S100P occurs under physiologically relevant conditions.
This IL-11 - S100P interaction warrants consideration in the development of anti-cancer drugs targeting IL-11 signaling pathways.