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Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

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Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches

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Small Molecule Active Site Directed Tools for Studying Human Caspases.

Marcin Poreba¹, Aleksandra Szalek¹, Paulina Kasperkiewicz¹

¹Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology , Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland.

Chemical Reviews

|November 10, 2015

Summary

Caspases are key regulators of cell death and inflammation. This review assesses technologies for studying caspase activity, including substrates, inhibitors, and activity-based probes, crucial for understanding diseases and developing drugs.

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Area of Science:

Biochemistry
Molecular Biology
Enzymology

Background:

Caspases are proteases involved in apoptosis and inflammation.
Dysregulation of caspases is linked to cancer, neurodegenerative, and autoimmune diseases.
Caspases are significant drug targets in academic and industrial research.

Purpose of the Study:

To systematically review technologies for investigating caspase activity and specificity.
To highlight the utility of substrates, inhibitors, and activity-based probes.
To provide a comprehensive assessment of tools for caspase research.

Main Methods:

Literature review of technologies for caspase activity assessment.
Analysis of substrates, inhibitors, and activity-based probes.
Evaluation of methods for studying caspase specificity and biological functions.

Main Results:

Various technologies exist for studying caspases, including traditional substrates and inhibitors.
Activity-based probes have emerged as powerful tools for investigating caspase functions.
These methods are essential for understanding disease mechanisms and drug development.

Conclusions:

A comprehensive understanding of caspase activity is vital for disease research.
Activity-based probes offer unique advantages for studying caspase biology.
Continued technological development is crucial for advancing caspase-targeted therapies.