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Published on: April 18, 2025
VEGFR-1 activation-induced MMP-9-dependent invasion in hepatocellular carcinoma
Tao Li1, Yuhua Zhu1, Lihui Han2
1Department of Infectious diseases, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Aim:
VEGFR-1 can promote invasion through epithelial-mesenchymal transition induction in hepatocellular carcinoma (HCC). This study aims to elucidate VEGFR-1 impact on proteolytic enzymes profile involved with invasion.
Materials & Methods:
The effect on cell invasion was evaluated by invasive and migration assays with and without VEGFR-1 activation. The mechanism was investigated by real-time PCR, western blot and gelatin zymography using inhibitors for MMP-9. In total, 95 HCC patients were enrolled for its clinical value evaluation.
Results:
VEGFR-1 activation induced invasion in HCC cells with an increase in the expression and activity of MMP-9 and Snail. MMP-9 blockage effectively inhibited VEGFR-1-induced invasion. High coexpression of both in HCC predicted a worse clinical outcome.
Conclusion:
Data show a novel VEGFR-1 activation-to-MMP-9 mechanism promoting HCC invasion.
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