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Expression of the plum pox virus coat protein region in Escherichia coli
D Mattanovich1, G Himmler, M Laimer
1Institute for Applied Microbiology, University of Agriculture, Vienna, Austria.
Virus Genes
|March 1, 1989
Abstract:
A cDNA complementary to the 3' end of plum pox virus (PPV) RNA was sequenced. The sequence was investigated for the presumable coat protein cistron by computer-aided translation. A fragment containing the stop codon of the polyprotein gene and a putative virus-specific protease cleavage site was subcloned into an E. coli expression vector. It is shown by immunological analysis that the coat protein cistron is located within the subcloned region.