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Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
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Cleaved Slit directs embryonic muscles.

Elly Ordan1, Talila Volk1

  • 1a Department of Molecular Genetics ; Weizmann Institute of Science ; Rehovot , Israel.

Fly
|November 12, 2015
PubMed
Summary

Slit cleavage immobilizes Slit signaling on tendon cells, guiding muscle elongation. Robo2 facilitates this cleavage, creating a stop signal for approaching muscles via Slit-N oligomers.

Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Molecular Biology

Background:

  • Musculoskeletal system formation requires precise muscle-tendon connections.
  • Drosophila larval muscles elongate towards tendons during embryogenesis.
  • The Slit/Robo signaling pathway is involved in muscle elongation towards tendons.

Purpose of the Study:

  • To investigate the role of Slit cleavage in muscle-tendon connectivity.
  • To elucidate the mechanism of Slit signal stabilization on tendon cells.
  • To understand how Slit signaling regulates muscle elongation arrest.

Main Methods:

  • Analysis of Slit cleavage products (Slit-N and Slit-C).
  • Investigation of Slit-N oligomerization and cell surface association.
Keywords:
RoboSlitSlit cleavagemusclemyotendinous jucntion

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  • Examination of Robo2's role in facilitating Slit cleavage.
  • Study of Slit-N binding to muscle receptors (Robo, Robo3).

    • Main Results:

    • Slit cleavage produces stable Slit-N and rapidly degrading Slit-C.
    • Slit-N oligomerizes and stabilizes on tendon cell surfaces.
    • Robo2 co-expressed by tendon cells facilitates Slit cleavage.
    • Cleaved Slit-N acts as a short-range repellent, arresting muscle elongation.

    Conclusions:

    • Slit cleavage is critical for immobilizing and stabilizing Slit signals on tendon cells.
    • Robo2-dependent Slit cleavage and Slit-N oligomerization direct muscle elongation.
    • The Slit-N signal provides a stop cue for approaching muscles via Robo and Robo3 receptors.