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Updated: Mar 30, 2026

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes
Published on: May 19, 2019
Deficiency of respiratory chain complex I in Hashimoto thyroiditis
Franz A Zimmermann1, Daniel Neureiter2, René G Feichtinger1
1Department of Pediatrics, Paracelsus Medical University, Müllner Hauptstraße 48, A-5020 Salzburg, Austria; Research Program for Receptor Biochemistry and Tumor Metabolism, Paracelsus Medical University, Müllner Hauptstraße 48, A-5020 Salzburg, Austria.
Abstract:
Oncocytic cells (OCs) are characterized by an accumulation of mitochondria and their occurrence in the thyroid gland of patients with Hashimoto thyroiditis (HT) is well known. However, their properties and functional relevance are poorly understood. We investigated OC lesions (n=212) in the thyroid of 12 HT patients. Loss of complex I protein was observed in oncocytic lesions of each of the patients. In addition to isolated complex I deficiency, 25% of oncocytic lesions showed combined deficiency of complex I and IV. Thus, we demonstrate for the first time a defect of respiratory chain complex I in OCs of HT patients.
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