Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

3.9K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.9K
MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

24.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cobalt-Catalyzed Intramolecular Dearomative Alkylation of Phenols with Alkenes.

Organic letters·2026
Same author

Polyoxygenated cembrane-type diterpenes from the Hainan soft coral Lobophytum crassum as a promising source of anticancer agents with ErbB3 and ROR1 inhibitory potential.

Acta pharmacologica Sinica·2024
Same author

Efficacy, immunogenicity, and safety of a monovalent mRNA vaccine, ABO1020, in adults: A randomized, double-blind, placebo-controlled, phase 3 trial.

Med (New York, N.Y.)·2024
Same author

In Vitro Insights into the Role of 7,8-Epoxy-11-Sinulariolide Acetate Isolated from Soft Coral <i>Sinularia siaesensis</i> in the Potential Attenuation of Inflammation and Osteoclastogenesis.

Marine drugs·2024
Same author

Neutralization of Omicron XBB.1 by booster vaccination with BA.4/5 monovalent mRNA vaccine.

Cell discovery·2024
Same author

Four New Diterpenoids from the South China Sea Soft Coral <i>Sinularia nanolobata</i> and DFT-Based Structure Elucidation.

Molecules (Basel, Switzerland)·2023

Related Experiment Video

Updated: Mar 30, 2026

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

26.3K

Exosomes decrease sensitivity of breast cancer cells to adriamycin by delivering microRNAs.

Ling Mao1,2, Jian Li1,3, Wei-Xian Chen1,3

  • 1The Fourth Clinical School of Nanjing Medical University, Nanjing, China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|November 12, 2015
PubMed
Summary

Adriamycin-resistant breast cancer cells release exosomes carrying microRNAs (miRNAs) that transfer drug resistance to sensitive cells. This exosome-mediated miRNA transfer promotes a pro-resistance tumor microenvironment.

Keywords:
AdriamycinBreast cancerChemotherapyDrug resistanceExosomesMicroRNAs

More Related Videos

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
08:49

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

11.8K
Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

844

Related Experiment Videos

Last Updated: Mar 30, 2026

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

26.3K
Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
08:49

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

11.8K
Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

844

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • Adriamycin (ADR) resistance is a major challenge in breast cancer (BCa) treatment.
  • Exosomes facilitate intercellular communication by transferring microRNAs (miRNAs) between cancer cells.
  • Previous studies indicated exosome-derived miRNAs from docetaxel-resistant BCa cells modulate chemosensitivity.

Purpose of the Study:

  • To investigate the role of exosome-mediated miRNA delivery in adriamycin resistance transmission in BCa.
  • To determine if exosomes from adriamycin-resistant BCa cells transfer miRNAs and confer resistance to sensitive cells.

Main Methods:

  • Isolation and characterization of exosomes (A/exo) from adriamycin-resistant BCa cells.
  • Co-culture of A/exo with adriamycin-sensitive BCa cells (GFP-S).
  • Quantification of miRNA transfer and analysis of gene expression changes in recipient cells.

Main Results:

  • Adriamycin-resistant BCa cells selectively package specific miRNAs into exosomes (A/exo).
  • Upregulation of A/exo-derived miRNAs was observed in recipient sensitive cells (GFP-S).
  • Gene ontology analysis revealed A/exo miRNAs target crucial biological processes, including the Wnt signaling pathway.
  • Co-culture with A/exo increased adriamycin resistance and altered gene expression in sensitive BCa cells.

Conclusions:

  • Exosomes derived from adriamycin-resistant BCa cells transmit drug resistance by delivering specific miRNAs.
  • Exosome-mediated miRNA transfer contributes to a pro-resistance tumor microenvironment by altering gene expression in sensitive cells.
  • Targeting exosome communication may offer a novel strategy to overcome adriamycin resistance in breast cancer.