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Related Experiment Videos

Receptor diffusion on cell surfaces modulated by locally bound concanavalin A.

J Schlessinger, E L Elson, W W Webb

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1977
    PubMed
    Summary

    Platelet binding to mouse fibroblasts globally inhibits cell surface receptor mobility, supporting the anchorage modulation hypothesis. This effect is distance-independent and partially reversible with microtubule-disrupting agents.

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    Area of Science:

    • Cell Biology
    • Biophysics
    • Molecular Biology

    Background:

    • Cell surface receptor mobility is crucial for cellular functions.
    • The anchorage modulation hypothesis proposes that receptor mobility can be globally regulated by local interactions.
    • Understanding these mechanisms is key to deciphering cell signaling and adhesion.

    Purpose of the Study:

    • To test the anchorage modulation hypothesis.
    • To quantify the effect of localized platelet binding on global cell surface receptor mobility.
    • To investigate the role of cytoskeletal components in this modulation.

    Main Methods:

    • Utilized fluorescence photobleaching recovery (FPR) to measure receptor diffusion.
    • Labeled 3T3 mouse fibroblasts and platelets with concanavalin A (Con A).

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  • Analyzed immobile and mobile receptor populations and their diffusion coefficients.

    • Main Results:

    • Con A-platelet binding decreased the diffusion coefficient of mobile receptors by a factor of 6.
    • The modulation effect was distance-independent from binding sites, indicating a global phenomenon.
    • Microtubule-disrupting agents partially reversed the observed mobility changes.

    Conclusions:

    • Findings support the anchorage modulation hypothesis.
    • Demonstrated a global, propagated inhibition of receptor mobility induced by localized platelet binding.
    • Highlighted the involvement of submembranous cytoskeletal elements (microtubules) in regulating receptor mobility.