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Updated: Mar 30, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
Construction, Enzyme Response, and Substrate Capacity of a Hyaluronan-Cyclodextrin Supramolecular Assembly
Ping Hu1, Yong Chen1,2, Jing-Jing Li1
1Department Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, 300071, P.R. China.
Abstract:
A supramolecular assembly was constructed with a cationic cyclodextrin (EICD) and native hyaluronan (HA). The cationic carboxylic ester pendants on HA support hyaluronidase (HAase)-responsive sites and the EICD supports artificial carboxylic esterase responsive sites. Substrate-binding models were investigated by using environment-sensitive fluorescence probes 2-p-toluidino-6-naphthalenesulfoniate sodium (2,6-TNS) and thioflavin T (ThT). On a HA/EICD assembly, EICD was able to bind an anionic substrate and HA and EICD constructed the cationic substrate binding site together. This assembly could be used as a sequential dual-substrate carrier.
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