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Human Virome01:26

Human Virome

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The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible...
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Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
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Probing the Viromic Frontiers.

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A new virus, human hepegivirus-1 (HHpgV-1), was discovered using high-throughput sequencing (HTS). A novel platform, VirCapSeq-VERT, enables sensitive detection of known and uncharacterized viruses, improving public health and blood safety.

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Area of Science:

  • Virology
  • Molecular Biology
  • Bioinformatics

Background:

  • High-throughput sequencing (HTS) has transformed virus discovery and virome analysis.
  • The family Flaviviridae includes important human pathogens.
  • Detecting novel viruses is crucial for public health and diagnostics.

Purpose of the Study:

  • To identify and characterize novel viruses within the Flaviviridae family.
  • To develop a sensitive, unbiased screening method for detecting known and uncharacterized viruses.
  • To assess the implications of a newly discovered virus for public health and blood supply safety.

Main Methods:

  • Utilized high-throughput sequencing (HTS) to analyze viral sequences.
  • Developed and applied the virome-capture-sequencing platform for vertebrate viruses (VirCapSeq-VERT).
  • Employed positive-selection oligonucleotide capture for sensitive viral sequence detection.

Main Results:

  • Identified a previously undescribed virus, human hepegivirus-1 (HHpgV-1), within the Flaviviridae family.
  • HHpgV-1 exhibits genomic features linking it to both hepaciviruses and pegiviruses.
  • VirCapSeq-VERT demonstrated high sensitivity in detecting low-abundance viral sequences, including HHpgV-1, in complex samples.

Conclusions:

  • HHpgV-1 may represent a new genus within the Flaviviridae family, with potential public health significance.
  • VirCapSeq-VERT offers a powerful new paradigm for detecting both known and novel viruses.
  • The findings underscore the importance of advanced sequencing technologies for comprehensive virome analysis and pathogen discovery.