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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Screening for Mupirocin Resistance in Staphylococcus

Avr Jeya Sanju1, Sridharan Sathyamoorthy Kopula2, Kennedy Kumar Palraj2

  • 1Student, Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University , Porur, Chennai, India .

Journal of Clinical and Diagnostic Research : JCDR
|November 12, 2015
PubMed
Abstract

Insights

Mupirocin resistance screening is crucial for Staphylococcus aureus and Coagulase-negative Staphylococcus (CoNS) infections. Disc diffusion may miss low-level mupirocin resistance, necessitating further testing for effective treatment.

Area of Science:

  • Clinical Microbiology
  • Antimicrobial Resistance
  • Dermatology

Background:

  • Mupirocin is a key topical antibiotic for Staphylococcus and Streptococcus infections.
  • Nasal mupirocin aids in eradicating methicillin-resistant Staphylococcus aureus (MRSA).
  • Increasing mupirocin usage has led to resistance and treatment failures.

Purpose of the Study:

  • To evaluate mupirocin resistance in Staphylococcus isolates.
  • To compare disc diffusion and minimum inhibitory concentration (MIC) methods for resistance detection.

Main Methods:

  • A cross-sectional study involving 100 Staphylococcus strains from skin infections.
  • Disc diffusion and agar dilution methods were used to determine mupirocin resistance.
  • Methicillin susceptibility was assessed using oxacillin and cefoxitin discs.

Main Results:

  • Mupirocin resistance detected in 12.5% of Staphylococcus aureus and 41% of CoNS by disc diffusion.
  • MIC testing revealed higher resistance rates: 19.6% for S. aureus and 43.1% for CoNS.
  • Coagulase-negative staphylococci (CoNS) exhibited higher mupirocin resistance than Staphylococcus aureus.

Conclusions:

  • Disc diffusion is a valuable initial screening tool for mupirocin resistance.
  • Mupirocin resistance is more prevalent in CoNS than S. aureus.
  • Disc diffusion may underestimate mupirocin resistance, particularly low-level resistance, highlighting the need for MIC testing.
Keywords:
High level mupirocin resistanceLow level mupirocin resistanceMethicillin resistant Staphylococcus aureus

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