Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.5K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.2K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.2K
RNA-seq03:21

RNA-seq

12.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.5K
Next-generation Sequencing03:00

Next-generation Sequencing

101.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
101.0K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

17.4K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
17.4K
Protein Complex Assembly02:41

Protein Complex Assembly

2.7K
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuronal type-specific modulation of cognition and AP-1 signaling by early-life rearing conditions.

Nature communications·2025
Same author

Deep learning approach for automatic assessment of schizophrenia and bipolar disorder in patients using R-R intervals.

PLoS computational biology·2025
Same author

AlfaPang: alignment free algorithm for pangenome graph construction.

Algorithms for molecular biology : AMB·2025
Same author

Sequence Flow: interactive web application for visualizing partial order alignments.

BMC genomics·2024
Same author

Assessment of symptom severity in psychotic disorder patients based on heart rate variability and accelerometer mobility data.

Computers in biology and medicine·2024
Same author

Effective modeling of the chromatin structure by coarse-grained methods.

Journal of biomolecular structure & dynamics·2024

Related Experiment Video

Updated: Mar 30, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

6.0K

RECORD: Reference-Assisted Genome Assembly for Closely Related Genomes.

Krisztian Buza1, Bartek Wilczynski1, Norbert Dojer1

  • 1Faculty of Mathematics, Informatics and Mechanics (MIM), University of Warsaw, Banacha 2, 02-097 Warsaw, Poland.

International Journal of Genomics
|November 12, 2015
PubMed
Summary

Researchers can now reconstruct closely related genomes using existing sequencing reads. This new method, RECORD, reconstructs modified reference genomes efficiently without extra sequencing costs.

More Related Videos

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.2K
Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.9K

Related Experiment Videos

Last Updated: Mar 30, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

6.0K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

9.2K
Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.9K

Area of Science:

  • Genomics
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) generates vast amounts of data, often exceeding the genome size.
  • Current protocols typically disregard variations within reads, relying solely on a reference genome.
  • This leads to a loss of valuable information about individual genome differences.

Purpose of the Study:

  • To develop a novel approach for reconstructing genomes closely related to a reference genome.
  • To enable the utilization of existing sequencing reads for generating modified reference sequences.
  • To introduce a cost-effective and rapid method for genome reconstruction.

Main Methods:

  • Application of de novo assembly software to experimental reads and pseudoreads.
  • Generation of contigs from assembled reads.
  • Creation of a modified reference sequence using the generated contigs.

Main Results:

  • The developed approach, implemented as RECORD software, reconstructs modified reference sequences with full coverage.
  • RECORD efficiently generates new reference sequences closer to the actual sequenced genome.
  • Evaluation on simulated and real data demonstrates RECORD's effectiveness.

Conclusions:

  • RECORD offers a significant advancement in genome reconstruction from NGS data.
  • The software outperforms general assisted-assembly tools for closely related sequences.
  • RECORD provides a rapid, cost-effective solution for researchers studying genome variations.