Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Southern Blot02:57

Southern Blot

24.5K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
24.5K
In-situ Hybridization02:31

In-situ Hybridization

11.0K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decarbonization pathways in medical waste management through circular economy strategies to advance UN-SDGs.

Scientific reports·2025
Same author

Enhanced separation of oil in water emulsions using PVDF composite membranes reinforced with graphene oxide doped titania and silica nanoparticles.

Scientific reports·2025
Same author

Optimized Congo Red Dye Adsorption Using ZnCuCr-Based MOF for Sustainable Wastewater Treatment.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

A flow cytometric approach to compare stem cell apoptosis in aplastic anemia and hypoplastic myelodysplastic syndrome.

Hematology/oncology and stem cell therapy·2024
Same author

Bio-spectroscopic analysis of corneal structural alterations in dry eye disease: A study of collagen, co-enzymes, lipids, and proteins with emphasis on phytotherapy intervention.

International journal of biological macromolecules·2024
Same author

Linagliptin Mitigates TGF-β1 Mediated Epithelial-Mesenchymal Transition in Tacrolimus-Induced Renal Interstitial Fibrosis via Smad/ERK/P38 and HIF-1α/LOXL2 Signaling Pathways.

Biological & pharmaceutical bulletin·2024

Related Experiment Video

Updated: Mar 30, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K

DNA Hybridization on Chitosan-Functionalized Silicon Substrate.

Amina Omar, El-Sayed M El-Sayed, Mona S Talaat

  • 1Spectroscopy Department, National Research Center, 33 El-Bohouth St. 12622 Dokki, Giza, Egypt. medahmed6@yahoo.com.

Medicinal Chemistry (Shariqah (United Arab Emirates))
|November 13, 2015
PubMed
Summary

This study uses computational methods to model DNA interactions on functionalized silicon surfaces. Findings enhance understanding of DNA hybridization for biosensor development in diagnostics and drug discovery.

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K
Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.4K

Related Experiment Videos

Last Updated: Mar 30, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.7K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K
Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.4K

Area of Science:

  • Biochemistry
  • Computational Chemistry
  • Materials Science

Background:

  • DNA hybridization is crucial for genetics, medical diagnostics, and drug discovery, forming the basis of biosensing devices.
  • Single-stranded DNA (ssDNA) probes immobilized on surfaces recognize complementary targets, creating stable duplexes for specific bio-recognition.

Purpose of the Study:

  • To computationally evaluate the interaction of aldehyde ssDNA on a chitosan-functionalized silicon substrate.
  • To assess the biological activity of proposed compounds using molecular modeling and Quantitative Structure Activity Relationship (QSAR) calculations.
  • To investigate target DNA interaction with a DNA probe on a chitosan-functionalized silicon substrate via hydrogen bonding.

Main Methods:

  • Utilized molecular modeling and Quantitative Structure Activity Relationship (QSAR) calculations at PM3 and MM3 levels.
  • Performed molecular modeling of ssDNA 5'-(TTCA) attached to a chitosan-functionalized silicon dioxide substrate.
  • Analyzed interactions through hydrogen bonding.

Main Results:

  • The study successfully modeled DNA interactions on functionalized silicon surfaces.
  • Evaluated the binding affinity and biological activity of the designed DNA probe system.
  • Provided insights into the molecular mechanisms of DNA hybridization for biosensing.

Conclusions:

  • Computational modeling and QSAR are effective tools for evaluating DNA-surface interactions.
  • The findings contribute to the rational design of advanced DNA-based biosensors.
  • This research supports the development of novel diagnostic and drug discovery tools.