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miR-490-5p suppresses tumour growth in renal cell carcinoma through targeting PIK3CA

Ke Chen1,2, Jin Zeng1,2, Kun Tang1,2

  • 1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.

Biology of the Cell
|November 13, 2015
PubMed
Abstract

Insights

MicroRNA-490-5p is downregulated in renal cell carcinoma, acting as a tumor suppressor by targeting PIK3CA. This finding suggests miR-490-5p as a potential therapeutic target for kidney cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are implicated in various human cancers, including renal cell carcinoma (RCC).
  • The specific role and expression of miR-490 in RCC progression were previously unknown.
  • Emerging evidence suggests miR-490's involvement in tumor development.

Purpose of the Study:

  • To investigate the expression profile of miR-490 in renal cell carcinoma.
  • To elucidate the functional role of miR-490 in RCC.
  • To identify the molecular targets and pathways regulated by miR-490 in RCC.

Main Methods:

  • Quantitative real-time PCR to assess miR-490-5p expression in RCC tissues and cells.
  • In vitro assays (e.g., cell proliferation, migration) and in vivo xenograft models to evaluate tumor suppressor activity.
  • Luciferase reporter assays and Western blotting to confirm direct binding of miR-490-5p to PIK3CA mRNA and its effect on protein levels.
  • Gene knockdown and overexpression studies to validate the mechanistic link between miR-490-5p and PIK3CA.

Main Results:

  • miR-490-5p expression was significantly downregulated in RCC tissues and cells compared to normal controls.
  • miR-490-5p demonstrated tumor suppressor functions in multiple in vitro and in vivo assays.
  • miR-490-5p directly targets the 3' UTR of PIK3CA mRNA, reducing PIK3CA expression and inhibiting the PI3K/Akt signaling pathway.
  • Knockdown of PIK3CA abolished the tumor-inhibitory effects of miR-490-5p, while PIK3CA overexpression reversed these effects.

Conclusions:

  • miR-490-5p acts as a tumor suppressor in renal cell carcinoma by targeting PIK3CA.
  • The miR-490-5p/PIK3CA axis plays a critical role in regulating RCC cell tumorigenicity.
  • miR-490-5p represents a potential therapeutic target for gene therapy in renal cell carcinoma treatment.
Keywords:
PIK3CARenal cell carcinomaTumour suppressormiR-490-5p

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