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Updated: Mar 30, 2026

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Published on: January 4, 2017
1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501
Christian Fercher1, Walter Keller1, Klaus Zangger2
1Institute for Molecular Biosciences, University of Graz, Humboldtstraße 50/III, 8010, Graz, Austria.
Abstract:
Conjugative transfer of DNA represents the most important transmission pathway in terms of antibiotic resistance and virulence gene dissemination among bacteria. TraH is a putative transfer protein of the type IV secretion system (T4SS) encoded by the Gram-positive (G+) conjugative plasmid pIP501. This molecular machine involves a multi-protein core complex spanning the bacterial envelope thereby serving as a macromolecular secretion channel. Here, we report the near complete (1)H, (13)C and (15)N resonance assignment of a soluble TraH variant comprising the C-terminal domain.
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