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Related Experiment Videos

Procedural guidelines for performing immunophenotyping by flow cytometry.

A L Landay1, K A Muirhead

  • 1Department of Immunology, Rush-Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.

Clinical Immunology and Immunopathology
|July 1, 1989
PubMed
Summary
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Flow cytometry is becoming standard for clinical immunophenotyping due to technological advances. However, quality assurance for this technique varies widely across laboratories, necessitating standardized procedures.

Area of Science:

  • Clinical Laboratory Science
  • Immunology
  • Biotechnology

Background:

  • Flow cytometry is increasingly adopted in clinical settings for cell analysis.
  • Advances in reagents, automation, and data analysis drive its use.
  • Current quality assurance practices for flow cytometry are inconsistent across laboratories.

Purpose of the Study:

  • To address the variability in quality assurance for clinical flow cytometry.
  • To propose consensus procedures for reliable immunophenotyping.
  • To highlight the need for standardized protocols in clinical flow cytometry.

Main Methods:

  • Discussion with a National Committee for Clinical Laboratory Standards (NCCLS) subcommittee.
  • Review of existing challenges and potential solutions for flow cytometry quality assurance.

Related Experiment Videos

  • Drafting of consensus procedures for immunophenotyping.

    • Main Results:

    • Identified significant variability in quality assurance (QA) procedures.
    • Developed proposed consensus procedures for flow cytometry immunophenotyping.
    • Highlighted the need for standardized QA to ensure reliable clinical results.

    Conclusions:

    • Standardized quality assurance is crucial for accurate clinical flow cytometry.
    • Consensus procedures are needed to overcome current QA variability.
    • The development of official NCCLS guidelines is anticipated.