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Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Subwavelength imaging through ion-beam-induced upconversion.

Zhaohong Mi¹, Yuhai Zhang², Sudheer Kumar Vanga¹

¹Department of Physics, Centre for Ion Beam Applications, National University of Singapore, Singapore 117542, Singapore.

Nature Communications

|November 13, 2015

Summary

This summary is machine-generated.

Researchers developed a novel subwavelength imaging method using lanthanide-doped nanocrystals and ionoluminescence. This technique overcomes optical diffraction limits for high-resolution cellular structure mapping.

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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

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Published on: August 30, 2017

Area of Science:

Nanotechnology
Biophysics
Microscopy

Background:

Optical microscopy is crucial for subcellular imaging but limited by Abbe's diffraction.
Conventional luminescent probes often degrade due to photobleaching, hindering long-term observation.
Existing techniques struggle to achieve resolutions below the diffraction limit.

Purpose of the Study:

To introduce a new subwavelength imaging method overcoming optical resolution limits.
To utilize lanthanide-doped upconversion nanocrystals for enhanced imaging capabilities.
To demonstrate a novel excitation source for inducing photon upconversion.

Main Methods:

Combining lanthanide-doped upconversion nanocrystals with ionoluminescence imaging.
Employing an ion beam as an excitation source for photon upconversion.
Experimental observation of ion beam-induced upconversion in nanocrystals.

Main Results:

Achieved subwavelength imaging with a spatial resolution below 30 nm.
Demonstrated simultaneous luminescence imaging and cellular structure mapping.
Validated the use of ion beams for exciting upconversion nanocrystals.

Conclusions:

The ionoluminescence imaging technique with lanthanide-doped nanocrystals offers a viable path to subwavelength biological imaging.
This method overcomes the limitations of traditional optical microscopy and photobleaching.
The developed approach provides high-resolution mapping of cellular structures for advanced biological studies.