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Potentiometric (bioselective electrodes) assay systems: utility and limitations.
1Department of Medicine, University of Tennessee, Memphis.
Critical Reviews in Clinical Laboratory Sciences
|January 1, 1989
Summary
Bioselective electrodes are transforming biotechnology with various potentiometric assays. This review details their utility, limitations, and future trends in biosensing applications.
Area of Science:
- Biotechnology
- Electrochemistry
- Biosensing
Background:
- Potentiometric assays using bioselective electrodes are rapidly advancing biotechnology.
- Electrochemical systems offer novel approaches for various analytical challenges.
Purpose of the Study:
- To review the utility and limitations of bioselective potentiometric assays.
- To provide an overview of concepts, history, and developments in biosensing.
Main Methods:
- Discussion of electrode architecture, including conventional liquid probes and solid-state transistor biosensors.
- Analysis of operational concepts, different biosensor types, and assay systems.
- Inclusion of advantages and disadvantages of various bioselective assay systems.
Main Results:
- Detailed examination of electrode design, chemoreceptors, biocatalysts, and potentiometric transducers.
- Evaluation of operational characteristics such as sensitivity, stability, and specificity.
- Coverage of enzyme, microbial, and immunoassay systems, including drug and microbial detection.
Conclusions:
- Bioselective potentiometric assays offer significant advantages but also have limitations that need consideration.
- Future trends in biosensing are highlighted, indicating continued innovation in the field.