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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Published on: October 15, 2013
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Polysilicon nanogap lab-on-chip facilitates multiplex analyses with single analyte.
Sharma Rao Balakrishnan1, U Hashim2, Subash C B Gopinath1
1Biomedical Nano Diagnostics Research Group, Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis (UniMAP), Kangar, Perlis, Malaysia.
Biosensors & Bioelectronics
|November 13, 2015
Summary
A novel polysilicon nanogap (PSNG) biosensor enables multiplex analysis for a single analyte, Human Chorionic Gonadotropin (hCG). The APTES-modified sensor demonstrated significantly higher sensitivity and a lower limit of detection for improved medical diagnostics.
Area of Science:
- Biosensing and Nanotechnology
- Medical Diagnostics
- Biomaterial Science
Background:
- Multiplex analyses are crucial for accurate medical diagnosis.
- Existing biosensing systems require improvement for single-analyte multiplexing.
- Polysilicon nanogap (PSNG) technology offers potential for advanced biosensing platforms.
Purpose of the Study:
- To design and fabricate a chemically functionalized PSNG lab-on-chip for multiplex analysis of a single analyte.
- To evaluate the performance of APTES and GPMS modified electrodes for Human Chorionic Gonadotropin (hCG) detection.
- To investigate the dielectric mechanisms underlying multiplex analysis for enhanced diagnostic accuracy.
Main Methods:
- Fabrication of a 69nm PSNG lab-on-chip.
- Chemical functionalization of PSNG electrodes with APTES and GPMS.
- Microfluidic delivery of hCG samples for single-injection analysis.
- Amperometric response and impedance measurements to assess analyte interaction.
- Evaluation of biocompatibility and structural characteristics for antibody binding.
Main Results:
- The PSNG biosensor demonstrated efficient binding of Human Chorionic Gonadotropin (hCG).
- APTES modification yielded 6.5 times higher sensitivity compared to GPMS.
- Achieved limit of detection of 0.56mIU/ml (APTES) and 2.93mIU/ml (GPMS).
- Dissociation constants (Kd) were determined as 5.65±2.5mIU/ml (APTES) and 7.28±2.6mIU/ml (GPMS).
Conclusions:
- The developed PSNG biosensor facilitates accurate multiplex analysis of a single target analyte.
- APTES modification enhances sensitivity and lowers the limit of detection for hCG.
- The system is simple, feasible, requires low sample volume, and is applicable for real-time comparative analyses.
