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Updated: Mar 30, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Octave-spanning hyperspectral coherent diffractive imaging in the extreme ultraviolet range.
High harmonic generation (HHG) enables tabletop soft x-ray microscopy for chemical analysis. This technique uses broad bandwidth light for chemically-specific hyperspectral imaging, overcoming previous limitations.
Area of Science:
- Physics
- Chemistry
- Materials Science
Background:
- Soft x-ray microscopy offers sub-micron resolution and chemical specificity via NEXAFS.
- Carbon K-edge (280-300 eV) provides high contrast for biological samples.
- Traditional soft x-ray imaging relies on large synchrotron or XFEL facilities.
Purpose of the Study:
- To demonstrate chemically-specific hyperspectral imaging using tabletop high harmonic generation (HHG) sources.
- To overcome the limitation of broad bandwidth in HHG for imaging applications.
- To develop a compact and versatile soft x-ray microscopy technique.
Main Methods:
- Utilized an octave-spanning HHG source producing coherent radiation up to 30 eV.
- Applied Fourier transform spectroscopy and lensless holographic imaging.
- Employed an interferometric delay reference to correct for source fluctuations.
Main Results:
- Achieved simultaneous multi-wavelength imaging of an object.
- Demonstrated the utility of broad HHG bandwidth for hyperspectral imaging.
- Showcased the potential for tabletop soft x-ray microscopy.
Conclusions:
- Tabletop HHG sources can be effectively used for advanced soft x-ray microscopy.
- Hyperspectral imaging is feasible with broad bandwidth HHG sources.
- This approach offers a promising alternative to large-scale facilities for chemical imaging.
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