Nutrient removal, microalgal biomass growth, harvesting and lipid yield in response to centrate wastewater loadings

The effects of wastewater, with four different nutrient loadings, from synthetic centrate on biomass production, nutrient removal, microalgal settling, and lipid production were investigated in photobioreactors under both batch and, subsequently, semi-continuous operations. At higher centrate concentration factors (17.2% and 36.2%), hydraulic retention time and pH adjustments could be employed to sustain acceptable microalgal growth rates and wastewater treatment. Similar nutrient removals efficiencies (>95%) and biomass production (0.42-0.51 g/L) were observed for the four centrate concentrations. Both the lipid productivity and lipid content decreased with increasing nutrient loading in the wastewater. The results also demonstrated that the mass ratio of carbohydrate to protein could provide a good indication of microalgal settling performance, rather than sole component composition or total extracellular polymeric substances. The highest settling efficiency (42.3 ± 0.04% after 24 h) and lowest lipid content (10.2 ± 1.6%) were observed for the lowest mass ratio of carbohydrate to protein (0.74 ± 0.15) noted in the microalgae cultivated in the wastewater with the highest centrate concentration factor (36.2%).