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Updated: Mar 30, 2026
Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
[Screening for mutants with thermostabe lipase A from Burkholderia sp. ZYB002]
Objective:
We improved the thermostability of LipA from Burkholderia cecapia ZYB002 by protein engineering technology to expand the application of lipase LipA.
Method:
On the basis of B-factor value of lipase LipA, series of potential mutation hotspots were selected for iterative saturation mutagenesis and the corresponding small mutation gene libraries were then constructed to screen the hyperthermal variants.
Results:
From the above mutation libraries, we obtained a series of mutants whose enzyme half-life at 55 degrees C increased by 1.7 to 2.2-fold.
Conclusion:
B-factor iterative test (B-FIT) is feasible to mutate thermostable strains.
