Decolorization of Sulphonated Azodye Metanil Yellow by Newly Isolated Bacterial Strain: Bacillus -3330

Area of Science:

• Environmental Microbiology
• Bioremediation
• Industrial Biotechnology

Background:

• Azo dyes are widely used in industries but pose environmental risks due to their persistence and toxicity.
• Effective and eco-friendly methods for dye decolorization are crucial for wastewater treatment.
• Microbial decolorization offers a sustainable approach to degrading recalcitrant dye compounds.

Purpose of the Study:

• To screen and evaluate the decolorization potential of a newly isolated bacterial strain, Bacillus sp. MTCC-3330.
• To investigate the efficacy of Bacillus sp. MTCC-3330 in decolorizing the sulfonated azo dye Metanil Yellow under aerobic conditions.
• To confirm dye degradation and assess the bacterium's ability to decolorize other azo dyes like Methyl Orange.

Main Methods:

• Isolation and screening of Bacillus sp. MTCC-3330 for dye decolorization.
• Aerobic decolorization experiments with Metanil Yellow at various concentrations (0.01mM, 0.02mM, 0.05mM).
• Monitoring decolorization using UV-VIS spectrophotometry to determine the maximum absorbance wavelength (λmax) and confirm dye degradation.

Main Results:

• Bacillus sp. MTCC-3330 achieved 100% decolorization of Metanil Yellow at 0.01mM, 0.02mM, and 0.05mM within 30, 35, and 45 hours, respectively.
• UV-VIS spectrophotometry confirmed dye degradation by the disappearance of the peak absorbance at 437 nm.
• The bacterium also demonstrated decolorization capabilities for another azo dye, Methyl Orange.

Conclusions:

• Bacillus sp. MTCC-3330 is a potent agent for the aerobic decolorization and degradation of Metanil Yellow.
• The study confirms the bacterium's ability to break down azo dyes, indicating its potential application in industrial wastewater treatment.
• This finding supports the use of microbial consortia or specific bacterial strains for eco-friendly dye effluent management.