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Related Experiment Videos

Nucleolin from Xenopus laevis: cDNA cloning and expression during development.

M Caizergues-Ferrer1, P Mariottini, C Curie

  • 1Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.

Genes & Development
|March 1, 1989
PubMed
Summary

Nucleolin, a key protein for ribosome biogenesis, was studied in Xenopus. Its expression during development suggests roles in both ribosome assembly and nucleologenesis.

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Nucleolin is a crucial nucleolar protein involved in ribosome biogenesis in eukaryotes.
  • Understanding nucleolin's function and evolution is key to comprehending cellular processes.

Purpose of the Study:

  • To investigate the presence, sequence, evolution, and expression of nucleolin in Xenopus laevis.
  • To elucidate the roles of nucleolin in ribosome biogenesis, nucleologenesis, oogenesis, and embryogenesis.

Main Methods:

  • Detection of nucleolin in Xenopus using an anti-hamster nucleolin antiserum.
  • Isolation and sequencing of a cDNA encoding Xenopus nucleolin.
  • Analysis of nucleolin expression patterns throughout oogenesis and embryogenesis.

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Main Results:

  • Xenopus nucleolin shares conserved domains with mammalian counterparts but shows distinct evolutionary paths, particularly in RNA-binding domains.
  • Nucleolin expression precedes rDNA transcription and ribosomal protein synthesis during early development.
  • Maximal nucleolin accumulation occurs during gastrulation, coinciding with nucleolar reformation.

Conclusions:

  • Nucleolin plays a significant role in both ribosome assembly and the formation of nucleoli (nucleologenesis).
  • The differential conservation of RNA-binding domains suggests specialized functions.
  • Nucleolin expression dynamics highlight its importance in developmental processes from oogenesis to embryogenesis.