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Updated: Mar 30, 2026
Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research
Published on: November 3, 2023
Tripterysium glycosides preconditioning attenuates renal ischemia/reperfusion injury in a rat model
Zhi-Shun Wang1, Tao Qiu2, Xiu-Heng Liu3
1Department of Urology, Renmin Hospital of Wuhan University, Wuhan University, ZiYang Road 99#, Wuhan, 430060, China. zhishun2005@126.com.
Background:
Ischemia-reperfusion (I/R) injury to the kidney occurs commonly in organ transplantation from donation after cardiac death, involving many pathologic processes. In this study, we used rat model to assess whether tripterysium glycosides (TG) preconditioning could exert protective effects in renal I/R injury.
Materials And Methods:
All male SD rats were randomly divided into four groups (6 each): sham group, TG group, I/R group and TG + I/R group. Groups TG and TG + I/R were pretreated with TG at 0.1 mg/kg for 14 days; groups sham and I/R were administered with the same dosage of normal saline. Groups TG + I/R and I/R underwent 45 min of renal ischemia of left kidney after right nephrectomy, and then, they were subjected to 72-h reperfusion. Groups sham and TG were only received right nephrectomy. The indicators of apoptosis, fibrosis and inflammation were analyzed to evaluate the effect of tripterysium glycosides preconditioning on renal I/R injury.
Results:
Pretreatment with TG significantly inhibited the levels of serum creatine and blood urea nitrogen and improved histologic lesions induced by I/R injury. Moreover, for the apoptosis signal pathway, pretreatment with TG markedly decreased the expression of caspase-3 and Bax and increased the level of Bcl-2. HMGB1, which was regarded as one of inflammation marker molecule, it was inhibited in the TG + I/R group. For the fibrosis signal pathway, the pretreatment with TG before I/R could down-regulate the expression level of typical molecules of fibrosis (TGF-β1, Smad3, p-Smad3).
Conclusions:
Pretreatment with tripterysium glycosides exhibited protective effect on kidney ischemia/reperfusion injury, which might be related to the alleviation of inflammation, fibrosis and the reduction in apoptosis.
Insights
Tripterysium glycosides (TG) preconditioning protected against kidney ischemia-reperfusion (I/R) injury in rats. This treatment reduced inflammation, fibrosis, and apoptosis, preserving kidney function and histology.
Area of Science:
- Nephrology
- Transplantation Immunology
- Pharmacology
Background:
- Kidney ischemia-reperfusion (I/R) injury is a common complication in organ transplantation.
- This injury involves complex pathological processes affecting kidney function.
- Donation after cardiac death increases the incidence of I/R injury.
Purpose of the Study:
- To investigate the protective effects of tripterysium glycosides (TG) preconditioning on renal I/R injury.
- To assess the impact of TG on apoptosis, fibrosis, and inflammation markers in a rat model.
Main Methods:
- Male Sprague-Dawley rats were divided into sham, TG, I/R, and TG + I/R groups.
- TG or saline was administered for 14 days prior to inducing renal ischemia (45 min) and reperfusion (72 h).
- Apoptosis, fibrosis, and inflammation indicators were analyzed.
Main Results:
- TG preconditioning significantly reduced serum creatinine and blood urea nitrogen levels.
- Histological lesions were improved, and apoptosis markers (caspase-3, Bax, Bcl-2) were modulated.
- Inflammation (HMGB1) and fibrosis (TGF-β1, Smad3) markers were significantly inhibited by TG.
Conclusions:
- Tripterysium glycosides demonstrate a protective effect against kidney I/R injury.
- The protective mechanism involves the alleviation of inflammation and fibrosis.
- TG preconditioning also reduces apoptosis, contributing to its renoprotective effects.
