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Updated: Mar 30, 2026
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
[Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice]
Qian-kun Zhu1, Gui-zhen He1, Hai-long Li1
1Department of Parenteral and Enteral Nutrition,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
Objective:
To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice.
Methods:
A total of 16 mice (C57BL/6J, specific pathogen free level) were randomly assigned into two groups (n=8 each): the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery.
Result:
The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion (P<0.05).
Conclusion:
The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.
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