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Updated: Mar 30, 2026

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
Published on: March 31, 2023
Biogenesis of autophagosomal precursors for LC3 lipidation from the ER-Golgi intermediate compartment
Liang Ge1, Livia Wilz1, Randy Schekman1
1a Department of Molecular and Cell Biology and Howard Hughes Medical Institute ; University of California ; Berkeley , CA USA.
Abstract:
Autophagosome biogenesis requires efficient mobilization and delivery of membranes from intracellular sources. How these membranes are mobilized remains poorly understood. Our recent work reported an autophagic signal-induced membrane mobilization event from the ER-Golgi intermediate compartment (ERGIC) to generate an early autophagosomal membrane precursor. We found that starvation activates the autophagic phosphatidylinositol 3-kinase, which promotes a relocation of COPII proteins from the ER-exit sites to the ERGIC. The relocation of COPII generates ERGIC-derived COPII vesicles as a membrane template for LC3 lipidation, a key step for autophagosome biogenesis.
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