Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.2K
Focusing of Light in the Eye01:16

Focusing of Light in the Eye

7.4K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
7.4K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.7K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

16.5K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
16.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Midline signaling regulates kidney positioning but not nephrogenesis through Shh.

Developmental biology·2010
Same author

Y chromosomal STR polymorphism in northern Chinese populations.

Biological research·2010
Same author

Construction of NF-κB-targeting RNAi adenovirus vector and the effect of NF-κB pathway on proliferation and apoptosis of vascular endothelial cells.

Molecular biology reports·2010
Same author

Hearing evaluation of intratympanic methylprednisolone perfusion for refractory sudden sensorineural hearing loss.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2010
Same author

Hydrothermal synthesis of nanostructures Bi12TiO20 and their photocatalytic activity on acid orange 7 under visible light.

Chemosphere·2010
Same author

An anticancer drug delivery system based on surfactant-templated mesoporous silica nanoparticles.

Biomaterials·2010

Related Experiment Video

Updated: Mar 30, 2026

Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging
05:45

Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging

Published on: January 19, 2024

1.6K

Lens-on-lens microstructures.

Qing Yang, Siyu Tong, Feng Chen

    Optics Letters
    |November 14, 2015
    PubMed
    Summary

    Researchers developed a novel method for fabricating lens-on-lens microstructures (LLMs) for advanced 3D imaging. This efficient technique enables precise control over focal lengths, crucial for real-time target detection.

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Microfabrication

    Background:

    • Microlenses with multiple focal lengths are essential for 3D imaging and dynamic target detection.
    • Existing fabrication methods may lack efficiency or scalability for complex micro-optical devices.

    Purpose of the Study:

    • To present a novel, efficient, and scalable method for fabricating lens-on-lens microstructures (LLMs).
    • To demonstrate the focusing and imaging capabilities of the fabricated LLMs.

    Main Methods:

    • Utilized a two-step femtosecond laser wet etching process.
    • Fabricated a 3x3 array of LLMs with a diameter of 129.0 μm.
    • Investigated control over size and focal length via laser power and etching time.

    More Related Videos

    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
    07:14

    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

    Published on: April 11, 2025

    1.4K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.3K

    Related Experiment Videos

    Last Updated: Mar 30, 2026

    Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging
    05:45

    Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging

    Published on: January 19, 2024

    1.6K
    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
    07:14

    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

    Published on: April 11, 2025

    1.4K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.3K

    Main Results:

    • Successfully fabricated LLMs with two distinct focal lengths (80.4 μm and 188.7 μm).
    • Demonstrated excellent two-level focusing and imaging performance.
    • Achieved a surface roughness of approximately 61 nm.

    Conclusions:

    • The developed femtosecond laser wet etching method is simple, efficient, and suitable for large-scale LLM production.
    • LLMs fabricated using this method show significant potential for diverse optical systems, including 3D imaging and real-time detection.