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Updated: Mar 30, 2026

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
Published on: February 17, 2017
Functional and structural characterization of a heparanase
Lisa Bohlmann1, Gregory D Tredwell1, Xing Yu1
1Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
Abstract:
We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.
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