Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structural Protein Function01:56

Structural Protein Function

3.4K
3.4K
Structural Protein Function01:56

Structural Protein Function

30.5K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<b>Special Volume of International Symposium of Tropical Fish Ecological Conservation and Biodiversity 2025 (ISTFECB 2025) Part I-Systematics, taxonomy, and species diversity of tropical fishes (Title page)</b>.

Zootaxa·2026
Same author

<b>Special Volume of International Symposium of Tropical Fish EcologicalConservation and Biodiversity 2025 (ISTFECB 2025) Part I-Systematics, taxonomy, and species diversity of tropical fishes (Table of contents)</b>.

Zootaxa·2026
Same author

<b>Preface: Special Volume of International Symposium of Tropical Fish Ecological Conservation and Biodiversity 2025 (ISTFECB 2025) Part I-Systematics, taxonomy, and species diversity of tropical fishes: contributed papers from ISTFECB 2025</b>.

Zootaxa·2026
Same author

<b>A new species of dwarf goby of <i>Trimma</i> (Teleostei: Gobiidae) from Taiping Island, South China Sea</b>.

Zootaxa·2026
Same author

<b>A new species of <i>Luciogobius</i> Gill (Teleostei: Gobiidae) from northern Taiwan</b>.

Zootaxa·2026
Same author

<b>A new wriggler of Eleotrid (Teleostei: Xenisthmidae) from Taiping Island, South China Sea</b>.

Zootaxa·2026

Related Experiment Video

Updated: Mar 30, 2026

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
10:41

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Published on: February 17, 2017

8.6K

Functional and structural characterization of a heparanase.

Lisa Bohlmann1, Gregory D Tredwell1, Xing Yu1

  • 1Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.

Nature Chemical Biology
|November 14, 2015
PubMed
Summary

Researchers characterized a novel heparanase from Burkholderia pseudomallei (BpHep). This study reveals its structure, function, and specificity for heparan sulfate, aiding inhibitor design.

More Related Videos

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
05:57

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

5.3K
Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating
05:35

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Published on: June 23, 2018

7.8K

Related Experiment Videos

Last Updated: Mar 30, 2026

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
10:41

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Published on: February 17, 2017

8.6K
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
05:57

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

5.3K
Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating
05:35

Surface Engineering of Pancreatic Islets with a Heparinized StarPEG Nanocoating

Published on: June 23, 2018

7.8K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Microbiology

Background:

  • Heparanases are enzymes that degrade heparan sulfate, playing roles in various biological processes.
  • Burkholderia pseudomallei is an invasive bacterial pathogen.
  • Understanding bacterial heparanases can provide insights into pathogenesis and potential therapeutic targets.

Purpose of the Study:

  • To structurally and functionally characterize a novel heparanase (BpHep) from Burkholderia pseudomallei.
  • To investigate the substrate specificity of BpHep.
  • To determine the first X-ray crystal structure of a heparanase.

Main Methods:

  • Protein expression and purification of BpHep.
  • Site-directed mutagenesis to identify active site residues.
  • Enzyme activity assays to determine substrate specificity.
  • X-ray crystallography to determine the 3D structure of BpHep.

Main Results:

  • BpHep shares approximately 24% sequence identity with human heparanase (hHep).
  • Key active site residues essential for enzymatic activity were identified.
  • BpHep exhibits specificity for heparan sulfate as a substrate.
  • The first X-ray crystal structure of a heparanase was determined.

Conclusions:

  • The structural and functional characterization of BpHep provides novel insights into heparanase mechanisms.
  • The determined crystal structure offers a basis for understanding substrate recognition.
  • This work lays the foundation for designing specific inhibitors against bacterial heparanases.