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Updated: Mar 30, 2026

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
Use of a mutant OGA for detecting O-GlcNAc modified proteins
1Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94143, U.S.A. chalkley@cgl.ucsf.edu.
Abstract:
In the previous issue of Biochemical Journal Mariappa et al. [(2015) Biochem J. 470,: 255-262] demonstrate a new method for visualizing O-linked N-acetylglucosamine (O-GlcNAc) modified proteins by making use of a catalytically dead version of the enzyme that normally removes this modification. They show their approach has broader specificity than current antibody-based techniques and higher specificity than lectin and chemical biology-based labelling approaches. This commentary discusses methods for O-GlcNAc detection and the significance of this work for characterizing this common, but currently poorly understood regulatory modification.
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