Related Experiment Video

Updated: Mar 30, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.8K

Involvement of FKBP6 in hepatitis C virus replication

Hirotake Kasai1, Kunihiro Kawakami2, Hiromasa Yokoe3

  • 1Department of Microbiology, Faculty of Medicine, University of Yamanashi, Chuo-shi, Yamanashi 409-3898, Japan.

Scientific Reports
|November 17, 2015
PubMed

Insights

Hepatitis C virus (HCV) uses the chaperone system for replication. Researchers found FKBP6, a cochaperone, is essential for HCV replication by interacting with the viral protein NS5A.

Area of Science:

  • Virology
  • Molecular Biology
  • Cellular Biology

Background:

  • Viruses hijack host cell machinery, including chaperone systems, for replication.
  • The hepatitis C virus (HCV) relies on host factors for its lifecycle.
  • Cochaperones play crucial roles in protein folding and viral processes.

Purpose of the Study:

  • To identify host factors essential for hepatitis C virus (HCV) replication.
  • To investigate the role of cochaperone FKBP6 in HCV replication.
  • To elucidate the interaction between FKBP6 and HCV proteins.

Main Methods:

  • Identified FKBP6 as an HCV replication factor.
  • Analyzed the interaction between FKBP6 and HCV nonstructural protein 5A (NS5A).
  • Utilized FKBP6-knockout hepatoma cell lines and FKBP8 inhibitors to assess viral replication.
  • Examined the colocalization of FKBP6, NS5A, FKBP8, and double-stranded RNA in infected cells.

Main Results:

  • FKBP6, a peptidyl prolyl cis-trans isomerase, interacts with HCV NS5A via its TPR domains and NS5A's Val(121).
  • FKBP6 colocalizes with NS5A, FKBP8, and double-stranded RNA in HCV-infected cells.
  • FKBP6 knockout cells showed complete suppression of HCV replication, which was restored by FKBP6 expression. FKBP8 inhibition also suppressed replication.

Conclusions:

  • FKBP6 is an HCV-induced host factor crucial for viral replication.
  • FKBP6 supports HCV replication by cooperating with NS5A and potentially FKBP8.
  • Targeting FKBP6 or its interaction with NS5A could be a therapeutic strategy against HCV.

More Related Videos

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors16:49

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Published on: July 16, 2012

12.1K
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.8K

Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver.
37
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
24
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.3K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.9K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.3K