Related Experiment Video

Updated: Mar 30, 2026

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
04:41

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

8.8K

Level of Blood Cell-Free Circulating Mitochondrial DNA as a Novel Biomarker of Acute Myocardial Ischemia

N P Sudakov1, T P Popkova, A I Katyshev

  • 1Irkutsk Surgery and Traumatology Research Center, Irkutsk, 664003, Russia.

Biochemistry. Biokhimiia
|November 17, 2015
PubMed

Insights

Elevated cell-free circulating mitochondrial DNA in blood indicates acute myocardial ischemia. This blood marker may help diagnose heart injury following adrenaline exposure.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Myocardial ischemia, a critical condition, requires accurate diagnostic biomarkers.
  • Cell-free circulating mitochondrial DNA (cf-mtDNA) is released during cellular damage.
  • Adrenaline-induced myocardial injury serves as an experimental model for studying heart damage.

Purpose of the Study:

  • To investigate the changes in blood cf-mtDNA levels during experimental adrenaline-induced myocardial injury in rats.
  • To assess the potential of cf-mtDNA as a biomarker for acute myocardial ischemia.

Main Methods:

  • Rats were subjected to subcutaneous adrenaline injections to induce myocardial injury.
  • Blood samples were collected at various time points post-injection.
  • Levels of cell-free circulating mitochondrial DNA in the blood were quantified.

Main Results:

  • A significant elevation in blood cf-mtDNA was observed at 48 and 72 hours after adrenaline administration.
  • This increase in cf-mtDNA correlated with the development of multiple small-focal myocardial ischemia.
  • Adrenaline-induced injury led to measurable changes in circulating mitochondrial DNA.

Conclusions:

  • Blood cf-mtDNA levels significantly increase in response to experimental myocardial ischemia.
  • Measured cf-mtDNA may serve as a valuable and sensitive biomarker for detecting acute myocardial ischemia.
  • Further research can explore the clinical utility of cf-mtDNA in diagnosing cardiac events.

More Related Videos

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

15.4K
Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes08:04

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Published on: August 16, 2021

3.0K

Related Concept Videos

Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
1.1K
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
742