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Related Experiment Video

Updated: Mar 30, 2026

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
08:49

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Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in

Ronghua Wu1, Yingying Yan2, Jian Yao3

  • 1Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China. wuronghua5@ntu.edu.cn.

International Journal of Molecular Sciences
|November 17, 2015
PubMed
Summary

Calpain 3 (CAPN3) protein levels fluctuate during muscle atrophy and regeneration after nerve injury. Lowering CAPN3 expression aids muscle recovery and promotes myoblast differentiation, suggesting a key role in skeletal muscle repair.

Keywords:
Calpain 3 (CAPN3)gastrocnemius musclemyoblast differentiationratsciatic nerve injury

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Area of Science:

  • Muscle physiology
  • Skeletal muscle biology
  • Regenerative medicine

Background:

  • Calpain 3 (CAPN3), a skeletal muscle protein, is implicated in muscular dystrophy.
  • The precise roles of CAPN3 in muscle atrophy and regeneration remain unclear.
  • Understanding CAPN3's function is crucial for developing therapeutic strategies for muscle disorders.

Purpose of the Study:

  • To investigate the effect of CAPN3 on muscle atrophy and regeneration following peripheral nerve injury.
  • To evaluate the expression pattern of active CAPN3 protein in rat gastrocnemius muscle post-sciatic nerve injury.
  • To elucidate the mechanistic role of CAPN3 in skeletal muscle recovery.

Main Methods:

  • Induction of reversible sciatic nerve injury in rats.
  • Measurement of gastrocnemius muscle wet weight ratio and cross-sectional area (CSA).
  • Analysis of active CAPN3 protein levels using Western blotting and correlation analysis.
  • CAPN3 knockdown using short interfering RNA (siRNA) and assessment of muscle recovery.
  • In vitro study of CAPN3 depletion on myoblast differentiation in L6 cells.

Main Results:

  • Muscle wet weight ratio and CSA decreased post-injury, with recovery observed later.
  • Active CAPN3 protein levels showed dynamic changes, decreasing early and increasing mid-stage.
  • Active CAPN3 protein expression was negatively correlated with muscle wet weight ratio.
  • CAPN3 knockdown via siRNA significantly improved muscle recovery at 7 and 14 days post-injury.
  • Depletion of CAPN3 promoted myoblast differentiation in L6 cells.

Conclusions:

  • Active CAPN3 protein expression is dynamically regulated during muscle atrophy and regeneration after denervation.
  • Early upregulation of CAPN3 may inhibit differentiation, potentially aiding satellite cell renewal.
  • Later downregulation of CAPN3 appears to stimulate myogenic differentiation, enhancing recovery.
  • CAPN3 plays a critical role in skeletal muscle regeneration, offering a potential therapeutic target.