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Serologic properties of the triple antibody-sandwich-lymphocyte-agglutination assay (TASLA)
C W Hewitt1, K S Black, L E Henson
1Department of Surgery, University of California, Irvine.
Detailed studies concerning the serologic properties of the triple antibody-sandwich-lymphocyte-agglutination (TASLA) assay are described herin. The technique is a sensitive one based on sandwiching three layers of antibody onto the target cell. Two different test systems were utilized which included xeno- and allogeneic models. In the xenogeneic test system, rabbit-anti-DA lymphocyte xenosera served as the primary antibody sandwich layer. Goat-anti-rabbit and swine-anti-goat IgG served as the secondary and tertiary antibody sandwich layers, respectively. In the rat allogeneic test system, LEW-anti-BN rat lymphocyte allosera served as the primary antibody layer. Rabbit-anti-rat and goat-anti-rabbit IgG served as the secondary and tertiary antibody sandwich layers, respectively. Several different experiments were run with varying numbers of antibody sandwich layers, and differing concentrations within each layer. The lymphocyte agglutination reaction was then evaluated by regression analysis. Regardless of the number or concentration of antibody sandwich layers, it was found that the reaction could be functionally defined mathematically, by regression analysis. A secondary or tertiary antibody sandwich layer increased assay sensitivity. The level of lymphocyte agglutination was found to be both a linear function of the number of antibody sandwich layers and the concentration of each utilized. In addition, the serological properties of the TASLA assay were extended to the rat allogeneic test system and was again functionally defined mathematically by regression analysis.
Detailed studies concerning the serologic properties of the triple antibody-sandwich-lymphocyte-agglutination (TASLA) assay are described herin. The technique is a sensitive one based on sandwiching three layers of antibody onto the target cell. Two different test systems were utilized which included xeno- and allogeneic models. In the xenogeneic test system, rabbit-anti-DA lymphocyte xenosera served as the primary antibody sandwich layer. Goat-anti-rabbit and swine-anti-goat IgG served as the secondary and tertiary antibody sandwich layers, respectively. In the rat allogeneic test system, LEW-anti-BN rat lymphocyte allosera served as the primary antibody layer. Rabbit-anti-rat and goat-anti-rabbit IgG served as the secondary and tertiary antibody sandwich layers, respectively. Several different experiments were run with varying numbers of antibody sandwich layers, and differing concentrations within each layer. The lymphocyte agglutination reaction was then evaluated by regression analysis. Regardless of the number or concentration of antibody sandwich layers, it was found that the reaction could be functionally defined mathematically, by regression analysis. A secondary or tertiary antibody sandwich layer increased assay sensitivity. The level of lymphocyte agglutination was found to be both a linear function of the number of antibody sandwich layers and the concentration of each utilized. In addition, the serological properties of the TASLA assay were extended to the rat allogeneic test system and was again functionally defined mathematically by regression analysis.