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09:10
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
Published on: October 28, 2019
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Function from within: Autophagy induction by HPSE/heparanase--new possibilities for intervention.
Neta Ilan1, Anna Shteingauz1, Israel Vlodavsky1
1a Cancer and Vascular Biology Research Center; the Bruce Rappaport Faculty of Medicine ; Technion ; Haifa , Israel.
Autophagy
|November 17, 2015
Summary
Heparanase (HPSE) promotes cancer growth and metastasis by cleaving heparan sulfate. This study reveals HPSE modulates autophagy, enhancing tumor cell growth and chemotherapy resistance.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Heparanase (HPSE) is a mammalian enzyme that degrades heparan sulfate.
- HPSE activity is linked to cancer cell invasion, metastasis, and reduced patient survival.
- The precise mechanisms of HPSE's role in tumor expansion remain unclear.
Purpose of the Study:
- To investigate the role of heparanase in cancer progression.
- To elucidate the mechanisms by which HPSE influences tumor growth and metastasis.
- To determine the impact of HPSE on cellular autophagy and chemotherapy resistance.
Main Methods:
- Analysis of HPSE expression in various cancer types.
- Investigation of HPSE's enzymatic activity on heparan sulfate.
- Assessment of autophagy modulation by HPSE in normal and malignant cells.
- Evaluation of chemotherapy resistance in cells with altered HPSE levels.
Main Results:
- HPSE expression is elevated in numerous cancers, correlating with metastasis and poorer outcomes.
- HPSE was found to modulate autophagy in both normal and cancerous cells.
- HPSE-mediated autophagy modulation confers growth advantages and enhances resistance to chemotherapy.
Conclusions:
- Heparanase plays a significant role in cancer progression by influencing autophagy.
- Targeting HPSE-mediated autophagy may offer a novel therapeutic strategy for overcoming chemotherapy resistance.
- Further research into HPSE's mechanisms could lead to improved cancer treatments.
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