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Related Experiment Video

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Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

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Published on: November 14, 2018

Finding NEMO in preeclampsia.

Agata Sakowicz¹, Paulina Hejduk¹, Tadeusz Pietrucha¹

¹Department of Medical Biotechnology, Medical University of Lodz, Poland.

American Journal of Obstetrics and Gynecology

|November 17, 2015

Summary

Increased NEMO gene expression in maternal and fetal blood is linked to preeclampsia development. Conversely, lower placental NEMO gene expression may cause apoptosis in preeclamptic pregnancies, offering new insights into this condition.

Keywords:

NEMO gene preeclampsia real-time reverse transcription–polymerase chain reaction

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Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Published on: November 20, 2015

Area of Science:

Genetics and Molecular Biology
Reproductive Medicine
Immunology

Background:

Preeclampsia's etiology and inheritance remain unclear.
Elevated pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) are observed in preeclampsia.
Nuclear factor-kappa B (NF-κB) pathway, regulated by NEMO (IKBKG), influences genes involved in cell cycle, proliferation, differentiation, and apoptosis.

Purpose of the Study:

To investigate the association between NEMO gene expression levels and preeclampsia.
To test the hypothesis that increased maternal and fetal NEMO gene expression contributes to preeclampsia.
To explore correlations between preeclampsia risk factors and NEMO gene expression in blood and placentas.

Main Methods:

Studied 91 women (43 preeclamptic, 48 controls) and their children.
Quantified NEMO mRNA levels (total and specific transcripts 1A, 1B, 1C) using real-time RT-PCR in maternal blood, cord blood, and placentas.
Performed univariate analyses and correlation tests to assess associations.

Main Results:

NEMO gene expression was approximately 7 times higher in preeclamptic women compared to controls.
A simultaneous increase in maternal and fetal NEMO mRNA expression correlated with preeclampsia.
Placental NEMO mRNA levels (total and transcripts) were significantly lower in preeclamptic pregnancies.

Conclusions:

Elevated NEMO gene expression in maternal and fetal blood appears relevant to preeclampsia development.
Reduced NEMO gene expression in preeclamptic placentas may contribute to increased apoptosis.
Findings suggest a potential role for NEMO in preeclampsia pathogenesis.