Related Experiment Video
Updated: Mar 30, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Quantitative determination of the antidepressant vortioxetine and its major human metabolite in plasma
Morten A Kall1, Morten Rohde1, Martin Jørgensen1
1Department of Bioanalysis, Drug ADME Research, H. Lundbeck A/S Ottiliavej 7-9, DK-2500 Valby-Copenhagen, Denmark.
Background:
Vortioxetine is a novel antidepressant that has been developed in a joint partnership between H. Lundbeck A/S and the Takeda Pharmaceutical Company, Ltd.
Results:
A number of bioanalytical methods have been developed in order to support the nonclinical and clinical development of the drug. Method performance, long-term stability, urine analysis, unspecific binding and metabolites analysis are presented and discussed.
Conclusion:
Two different method applications for the quantification of vortioxetine and its major human metabolite in human plasma, an isocratic cation exchange HPLC-MS/MS method utilizing C8-SPE sample extracts and a reversed-phase UPLC-MS/MS method with gradient elution of protein precipitated sample extracts, have been validated according to current regulatory standards and applied in support to a large number of nonclinical as well as clinical studies.
More Related Videos
12:21Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Published on: November 27, 2016
06:14Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients
Published on: October 15, 2017
Related Concept Videos
Antidepressant Drugs: MAOIs and Other Agents
Nonlinear Pharmacokinetics: Dependence of Elimination Half-Life and Dose Clearance
A study on guinea pigs examined the...
Measurement of Bioavailability: Pharmacodynamic Methods
Therapeutic Drug Monitoring: Drug Analysis Methods
Measurement of Bioavailability: Pharmacokinetic Methods
Antidepressant Drugs: Overview