Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhanced stability of immobilized xylanase with nano‑zinc oxide/ethyl cellulose composite carrier for efficient xylan hydrolysis.

Food chemistry·2026

Same author

Engineering a microbial platform for the biosynthesis of anthranilic acid and its derivatives.

Metabolic engineering·2026

Same author

Sequence redesign of glycosyltransferases for enhanced heterologous expression and glycosylation efficiency in Escherichia coli.

Nature communications·2026

Same author

Programmable methylation engineering: Design principles from alkaloid biosynthesis.

Biotechnology advances·2026

Same author

Corrigendum to "Establishing genetically stable Escherichia coli for highly effective myo-inositol production" [Bioresour. Technol. 443 (2026) 133874].

Bioresource technology·2026

Same author

Delving Into the Depths of <i>AGTR2</i>: In Silico Identification of Deleterious Nonsynonymous SNPs Associated With Cardiovascular Diseases.

Human mutation·2026

Search research articles

Related Experiment Video

Updated: Mar 30, 2026

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Published on: May 21, 2013

[Research progress in chondroitinase ABC].

Ye Li, Zhenya Chen, Qipeng Yuan

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|November 18, 2015

Summary

This summary is machine-generated.

Chondroitinase ABC (ChSase ABC) enzymes break down glycosaminoglycans (GAGs), enabling their study in vitro. This review covers ChSase ABC classification, structure, and applications, offering insights into future research directions.

More Related Videos

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

Published on: September 29, 2018

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

Related Experiment Videos

Last Updated: Mar 30, 2026

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Published on: May 21, 2013

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

Published on: September 29, 2018

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

Area of Science:

Biochemistry
Molecular Biology
Enzymology

Background:

Glycosaminoglycans (GAGs) are crucial extracellular matrix components regulating cell functions.
Large GAG molecular weights limit their in vitro applications.
Chondroitinase ABC (ChSase ABC) enzymes degrade GAGs, overcoming these limitations.

Purpose of the Study:

To review the classification and crystal structure of ChSase ABC.
To summarize recent research progress on ChSase ABCs.
To discuss methods for ChSase ABC separation, purification, and enzyme engineering.

Main Methods:

Literature review of ChSase ABC research.
Analysis of ChSase ABC classification and crystal structures.
Discussion of enzyme purification, stability, and immobilization techniques.

Main Results:

Detailed classification and structural insights into ChSase ABC.
Overview of methods for ChSase ABC production and characterization.
Analysis of ChSase ABC stability and immobilization strategies.

Conclusions:

ChSase ABC is a valuable tool for studying GAGs in vitro.
Advances in enzyme engineering and characterization are expanding ChSase ABC applications.
Future research should focus on overcoming current challenges and exploring novel applications.