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Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Published on: August 20, 2018
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Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins
Xu-Dong Wang1, Kersten S Rabe1, Ishtiaq Ahmed1
1Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz, 76344, Eggenstein-Leopoldshafen, Germany.
Advanced Materials (Deerfield Beach, Fla.)
|November 18, 2015
Abstract:
A convenient reverse micellar one-pot reaction yields multifunctional silica nanoparticles, which can be tailored to effectively suppress non-specific adsorption and, at the same time, enable efficient specific covalent immobilization of proteins. Using two highly sensitive proteins, it is demonstrated that the new particles provide a suitable microenvironment to maintain the protein's activity.
