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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
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A procoagulant chemically sulfated mannan.

Ana Helena P Gracher1, Aline G Santana1, Thales R Cipriani1

  • 1Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil.

Carbohydrate Polymers
|November 18, 2015
PubMed
Summary
This summary is machine-generated.

Chemically sulfated mannan acts as a procoagulant agent, enhancing blood coagulation and platelet aggregation. This polysaccharide shows potential as a hemostatic agent to prevent bleeding disorders.

Keywords:
Chemical analysisChemically sulfated polysaccharideMethylationProcoagulant effect

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Area of Science:

  • Biochemistry
  • Polymer Chemistry
  • Hematology

Background:

  • Disorders of hemostasis present significant clinical challenges.
  • Polysaccharides are investigated for their anticoagulant and procoagulant properties.
  • Mannan, a polysaccharide, was chemically modified to explore its hemostatic potential.

Purpose of the Study:

  • To chemically sulfate a specific mannan structure.
  • To characterize the sulfated mannan using advanced analytical techniques.
  • To evaluate the in vitro and in vivo effects of the sulfated mannan on blood coagulation.

Main Methods:

  • Chemical sulfation of mannan.
  • Structural characterization using Nuclear Magnetic Resonance (NMR) and Gas Chromatography-Mass Spectrometry (GC-MS).
  • In vitro and in vivo evaluation of blood coagulation and platelet aggregation.

Main Results:

  • High degree of substitution was confirmed on the sulfated polysaccharide.
  • The sulfated mannan demonstrated procoagulant activity, enhancing blood coagulation in normal and hemophilic plasma.
  • It also activated platelet aggregation and reduced activated partial thromboplastin time (aPTT) ex vivo.

Conclusions:

  • Sulfated mannan exhibits significant procoagulant properties.
  • This modified polysaccharide can increase blood coagulation and platelet aggregation.
  • Sulfated mannan represents a potential source for developing novel hemostatic agents to manage hemorrhagic conditions.