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Related Concept Videos

Protein-protein Interfaces02:04

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
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The interactome challenge.

John D Aitchison1, Michael P Rout2

  • 1Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), Seattle, WA 98109 Institute for Systems Biology, Seattle, WA 98109 rout@rockefeller.edu john.aitchison@cidresearch.org.

The Journal of Cell Biology
|November 18, 2015
PubMed
Summary
This summary is machine-generated.

Understanding the cell

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Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Cellular functions are driven by complex macromolecular interactions, collectively known as the interactome.
  • Dysregulation of these molecular interactions is a key factor in disease pathogenesis.

Purpose of the Study:

  • To highlight the critical need for advanced tools to study cellular interactomes.
  • To address the current limitations in dissecting the intricate network of molecular interactions within cells.

Main Methods:

  • This study reviews the current state of interactome research.
  • It emphasizes the necessity for developing novel methodologies and technologies.

Main Results:

  • Existing tools for interactome analysis are insufficient to fully capture dynamic cellular processes.
  • Significant advancements in technology are required to accurately map and understand molecular interactions.

Conclusions:

  • Improved tools are essential for a comprehensive understanding of cellular machinery and disease mechanisms.
  • The development of new technologies will enable deeper insights into the interactome and its role in health and disease.